Journal: Journal of Anatomy
Article Title: Callosal septa express guidance cues and are paramedian guideposts for human corpus callosum development
doi: 10.1111/joa.13011
Figure Lengend Snippet: To contrast the developmental maximum with the resolution of the septa, adjacent sections through the level of the callosal midbody septa were stained by the Nissl method (A,D), synaptophysin (B,E) and growth‐associated protein‐43 (GAP43) (C,F) immunohistochemistry, at 20 PCW (A–C) and 33 PCW (D‐F). Arrows point to the callosal septa. During the septal maximum, both synaptophysin and GAP43 immunoreactivity are confined within growing callosal axons and neither shows any immunoreactivity in any part of the septa (B,C). At 33 PCW, synaptophysin is expressed lightly within the tops of the septa, whereas GAP43‐immunopositivity is located through the full extent of the septa and partially in the subcallosal zone, indicating that the growing axons invade the septa, presumably leading to their resolution. Scale bars: 0.5 mm in (A–C) and 1 mm in (D,F).
Article Snippet: Qualitative analysis of the stained histological sections was performed using an upright Olympus Provis AX70 microscope, and images were captured with a Nikon DXM1200 digital camera. table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Primary antibodies (clone) Name or abbreviation (Cat. No.) Host, isotype Dilution Supplier Anti‐Microtubule‐Associated Protein‐2 (HM2) MAP2 (M4403) Mouse, monoclonal, IgG1, affinity purified hybridoma cell culture 1:1000 Sigma‐Aldrich, St. Louis, MO, USA Anti‐Microtubule‐Associated Protein‐1B (clone AA6) MAP1B (M4528) Mouse, monoclonal, IgG1, affinity purified hybridoma cell culture 1:1000 Sigma‐Aldrich, St. Louis, MO, USA Anti‐synaptophysin (SY38) synaptophysin (M0776) Mouse, monoclonal IgG1 kappa, cell culture supernatant 1:100 Dako, Glostrup, Denmark, EU Anti‐ synaptosomal‐associated protein 25 (SMI81) SNAP25 (836301) Mouse monoclonal IgG1, kappa, affinity purified hybridoma cell culture 1: 1000 Biolegend, San Diego, CA, USA Growth associated protein ‐43 GAP43 (NB300‐143) Rabbit polyclonal affinity purified IgG 1:1000 Novus Biologicals, LLC,Centennial, CO, USA Anti‐neogenin (N‐19) neogenin (sc‐6537) Goat polyclonal affinity purified IgG 1:200 Santa Cruz, Biotechnology, Inc. Dallas, TX, USA Anti‐ephrinA4 (P‐20) ephrinA4 (sc‐912) Rabbit polyclonal affinited purified IgG 1:200 Santa Cruz, Biotechnology, Inc. Dallas, TX, USA Anti‐human fibronectin fibronectin (F3648) Rabbit polyclonal, affinity purified antibody 1:400 Sigma‐Aldrich, St. Louis, MO, USA Anti‐Glial Fibrillary Acidic Protein GFAP (Z0334) Rabbit, polyclonal purified immunoglobulin 1:1000 Dako Glostrup, Denmark, EU Anti‐human CD68 (514H12) CD68 (MCA1815) Mouse, monoclonal IgG2a, tissue Culture Supernatant 1:80 Bio‐Rad Antibodies Kidlington, Oxford, UK Open in a separate window Primary antibodies used in the study Nissl‐stained sections at the level of the roof of the lateral ventricle were used to count the callosal septa.
Techniques: Staining, Immunohistochemistry
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